- Four (BSC3-4)
or six (BSC3-6) channel slice chamber
- Slices supported
on removable insert with nylon net
- Submerged and interface modes by adjusting
fluid level
- Proportional Temperature Controller (PTC03) with low noise
performance
This slice recording chamber is based on the BSC1 and BSC2 chambers
with both "interface" and "submerged" methods of maintaining slices. Four
or six separate chambers are machined on one acrylic block for simultaneous
perfusion with separate solution lines. As with BSC1 and BSC2 the temperature
is controlled by a proportional heating unit, the PTC03.
The chamber is constructed from clear acrylic having a diameter of 130mm,
overall height is 70mm and the base plate is 130mm in diameter. The slices
rest on a nylon net held in place by a close fitting removable insert.
Pre-oxygenated medium enters the main body of the chamber through a fine
bore tube which spirals in the heated distilled water in the lower part
of the chamber and enters the upper part of the chamber directly into
the feed for each of the six chambers. Depending on whether submerged
or interface type preparations are required, the height of the perfusion
fluid is adjusted at the exit well by means of a variable angle stainless
steel needle as with our MS-1 chambers. In the case of interface preparations,
the high oxygen tension is maintained by bubbling a 95% oxygen,
5% carbon dioxide gas mixture through a sintered bubbler located in the
lower heated part of the chamber. This moistened and warmed gas
mixture enters the upper part of the chamber via "port holes" and is then
deflected by a standard rectangular glass coverslip across the centrally
located slice preparation. The temperature in the upper chamber is maintained
by ensuring that the medium and moistened gas mixture enter at the required
temperature. This is dependent on the temperature of the lower chamber
body which is warmed by a heating element controlled by the Proportional
Temperature Controller, PTC03.
To download a flyer in PDF click here.
To download the instruction manual in PDF click here.
Schematic
diagram
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Copyright© 1999 Scientific
Systems Design, Inc.
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